Detailed Unification Equations for Kinetic Parameters: A Case Study on Human Salivary Alpha Amylase

There has been lot of interest in different kinds of rate constant which characterizes different enzymes [1-5]. Most enzymes studied may be described as industrial enzymes because of commercial objectives, which include production of industrial fuels, bioethanol [6-8], production of bulking agent, water retention agent, thickeners etc [4]. Some of the products are highly important in a wide range of nutritional, cosmetic and pharmaceutical applications [10-12]. The hydrolysis of starch from various sources is widely studied [12-16]. Most of the industrial enzymes studied are usually mesophiles and most often, thermophiles of microbial origin [17]. This research focuses mainly on a polymer called potato starch and human salivary alpha amylase which, has similar capacity and function as pancreatic amylase. It is not a misplacement to use salivary amylase (EC 3.2.1.1) as a model for this study because its normal activity is evidence of good health. It is a maker for various health indices [18,19]. It has been observed that direct entry of starch into the small intestine, by-passing partial digestion in the oral cavity results in substantially less starch digestion in the small intestine and glucose absorption [20]. These observations make it worthwhile to treat human salivary amylase exclusively.